The influence of culture media on chromosome aberration levels, sister chromatid exchange frequencies, and the rate of cell proliferation: comparison of Iscove's low folate medium with Eagle's MEM.
نویسندگان
چکیده
منابع مشابه
Sister chromatid exchange analysis in some Holstein bulls
Chromosomal appearance of 12 Holstein bulls selected for artificial inseminations were examined bysister chromatid exchanges (SCEs). For differential staining of sister chromatids bromodeoxyuridine (BrdU)was inoculated in lymphocyte cultures. The mean, maximum and minimum number of SCE per cell weredetermined 6.8 ± 1.14, 8.3 ± 1.1 and 5.7 ± 1.5, respectively. SCE frequencies of all animals were...
متن کاملStudy of the Effect of MMC on the Sister Chromatid Exchange in the Human Lymphocytes
Some environmental mutagenic agents cause genomic instability and increase susceptibility of DNA damage. One of them is mitomycin C which is connected to DNA as an alkylating factor and affects susceptible cells to reduction reactions. This drug is used in chemotherapy and treatment of tumors. Study of genomic instability in the presence of different concentrations of MMC can show susceptibilit...
متن کاملComparison of the effect of platelet lysate derived from umbilical cord blood and peripheral blood on the expansion and differentiation of MSCs
Abstract Background and Objectives Proliferation and differentiation of mesenchymal stem cells (MSCs) in culture media requires the presence of nutrient supplements such as bovine fetal serum (FBS), which increases the risk of prion/xenotic infections. Platelet lysates as a rich source of growth factors and cytokines can replace FBS in cell culture medium. This study aimed to compare the effe...
متن کاملO-31: Follicular Fluid The Best Medium for In Vitro Maturation and In Vitro Fertilization
Background: Induction of in vitro maturation and development of immature oocyte is one of the methods that has been frequently used recently in artificial reproduction techniques and has drawn attention of many investigators. This method is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment ...
متن کاملGenotoxic monitoring of workers at a hazardous waste disposal site in Mexico.
Chromosomal aberration and sister chromatid exchange (SCE) frequencies were determined in lymphocytes cultured from 12 high-risk individuals working at a landfill for hazardous waste disposal. Cell proliferation kinetics (CPK) was also determined. Compared with 7 control individuals, no effects were observed with respect to SCE nor on CPK. However, the workers exhibited significantly higher fre...
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عنوان ژورنال:
- Journal of medical genetics
دوره 25 6 شماره
صفحات -
تاریخ انتشار 1988